The microbiology laboratory is prioritized for practical work, research, and development in the field of microbiology. Activities commonly performed in this laboratory related to microbiology include:

• Sterilization and Disinfection Techniques for Laboratory Equipment
• Isolation, Identification, and Enumeration of Microbes and Fungi Obtained from Nature/Environment
• Testing the Antimicrobial and Antifungal Activities of Natural Materials
• Studying the Environmental Factors Affecting Microbial and Fungal Growth
• Investigating Metabolic Processes and Physiology of Microbes and Fungi
• Building a Collection of Pure Cultures with Characterized Microorganisms and Their Functions
• Developing Biotechnological Applications of Microorganisms in Food, Industry, Health, and Environmental Management, Emphasizing Local Wisdom in the Yogyakarta Community
• Observing and Developing Macroscopic Mushroom Cultivation
• To support these activities, the laboratory is equipped with a Macroscopic Mushroom Cultivation Unit.

The equipment used in this laboratory includes laminar airflow hoods (LAF), autoclaves, ovens, incubators, vortex mixers, microscopes, colony counters, water baths, hot plates, balances, refrigerators, spectrophotometers, centrifuges, tripods, wire gauze, micropipettes, Bunsen burners, microwaves, glassware, and more.

The practical courses offered in the Microbiology Laboratory unit include General Microbiology, Environmental Microbiology, Food and Industrial Microbiology, Mycology, and Phytopathology.