The Ecology Laboratory is utilized for practical activities, research, and development in the field of ecology. It encompasses terrestrial ecology (land), aquatic ecology (freshwater, brackish water, and saltwater), environmental issues, and conservation. The activities in this laboratory are closely tied to fieldwork. Some of the activities that can be conducted in the ecology laboratory include:

• Inventory of plants and animals in terrestrial and aquatic ecosystems (freshwater, brackish, and saltwater) in the Yogyakarta region.
• Exploration and management of terrestrial, coastal, and marine biological resources, as well as potential environmental changes.
• Analysis of environmental quality parameters such as water, soil, and air.
• Assessment of environmental damage and pollution, along with efforts for rehabilitation.
• Study of animal behavior and activities in their habitats.

The laboratory is equipped with environmental parameter measurement tools (physical and chemical) for both terrestrial and aquatic environments. Additionally, there are supporting tools for sample collection. Some of the ecological instruments include Thermometer, Thermohygrometer, Soil Tester, Anemometer, Barometer, Altimeter, Lux Meter, Hagameter, Secchi Disk, Refractometer, Turbidimeter, Graduated Scale Board, Conductivity Meter, DO Meter, Ekman Grab, Surber Net, Stacked Mesh Sieves, Garmin Maps GPS, Handycam, Hanna Instrument, Underwater Camera, Floating Dredge, Herbarium Kit, Plankton Net, Pipe Plot, Measuring Tape, Sample Bottles, Insect Net, Magnifying Glass, Compass, and others.

Furthermore, the practical courses offered in the Ecology Laboratory include Basic Ecology, Aquatic Ecology, Terrestrial Ecology, Animal Ecology, Aquaculture, Conservation Biology, and Ethnobiology